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Columns for synthetic oligonucleotides

8th December 2016

Posted By Paul Boughton


Phenomenex Inc introduces two new particle chemistries for the characterisation and purification of synthetic oligonucleotides.

Joining the company’s Clarity BioSolutions portfolio are Clarity Oligo-XT, for high-efficiency reversed-phase LC analysis and purification, and Clarity Oligo-SAX, a high-resolution strong anion exchanger (SAX) for characterisation.

Phenomenex Clarity BioSolutions, first introduced almost a decade ago, are used in pharmaceutical labs and contract research organisations (CROs) where synthetic oligonucleotide-based therapeutics are being developed and tested, as well as in the labs of custom oligonucleotide and nucleic acid manufacturers.

Synthetic oligonucleotides are growing in popularity as novel agents for the treatment of disease, and liquid chromatography continues to be an effective tool for characterisation, with technology advancements that address improvements in resolution, throughput and column lifetime.  

The new Clarity Oligo-XT C18 columns feature novel and robust core-shell media, for high-efficiency reversed-phase characterisation of synthetic DNA and RNA, pH stability from 1 to 12 and increased sensitivity that improves quantitation by mass spectrometry (MS).

These core-shell particles deliver the separation power necessary to accurately resolve closely related synthetic oligonucleotide sequences.

Clarity Oligo-XT columns are available in directly scalable 1.7µm, 2.6µm and 5µm particle sizes that enable easy method transfer between analytical HPLC/UHPLC instrumentation and preparative purifications systems.

The 5µm particle is available in Phenomenex’s patented Axia-packed preparative columns that increase purification performance.

The Clarity Oligo-SAX columns feature an entirely new, rugged non-porous particle that retains synthetic oligonucleotides through strong ion exchange mechanisms, adding a robust strong anion exchanger choice with improved column lifetimes to the Clarity family.

These quaternary amine functionalised, nonporous particles are engineered for performance at high pH (2.5 to 12.5) and temperatures up to 85˚C and are provided in 5µm particle size for analytical characterisation.





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