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Nano-ultra high performance liquid chromatography

1st April 2013


Thermo Fisher Scientific Inc says the Center for Experimental Bioinformatics (CEBI) at the University of Southern Denmark is using Thermo Scientific nano-ultra high performance liquid chromatography (UHPLC) technology to accelerate productivity and improve efficiencies during its proteomics research.

Implementation of the Thermo Scientific EASY-nLC 1000 has enabled 34 percent more peptide and 22 percent more protein identifications within the CEBI laboratory, significantly increasing productivity. Additionally, the instrument has improved separations and facilitated the analysis of more complex samples within the laboratory.

Based in Odense, the University of Southern Denmark’s Department of Biochemistry and Molecular Biology is world-renowned in the field of proteomics, with research focusing on gene structure and organization, regulation and RNA translation. CEBI is a specialist proteomics research laboratory, aimed at applying modern methods of mass spectrometry and proteomics to functional analysis of genes.

Prior to implementing nano-UHPLC technology, CEBI used conventional HPLC. However they needed a more complete solution, such as EASY-nLC 1000, to accelerate productivity and improve efficiencies. The EASY-nLC 1000 is used in conjunction with Thermo Scientific ion trap and Orbitrap mass spectrometers for a wide range of proteomics research, including the study of complex mouse dendritic cells.

Using the EASY-nLC 1000 has allowed CEBI to take advantage of the ability to facilitate dedicated separation of biomolecules at ultra-high pressures. The higher operating pressures provided by the EASY-nLC 1000 have increased the number of peptide and protein identifications performed within the laboratory by 34 percent and 22 percent respectively. The instrument also allows selection of the most appropriate column dimensions and solid phase materials in order to identify the most suitable configuration to match analytical requirements. Using thinner, longer columns and smaller
beads has improved separation and ionization, enhancing sensitivity and allowing the CEBI team to scale down experiments and reduce costs.

“The EASY-nLC 1000 is a highly robust instrument offering nano-UHPLC performance dedicated to proteomics scientists,” said Lasse Falkenby, research assistant at CEBI. “We decided to implement the new system in our laboratory as we felt that it would revolutionise the way we perform LC-MS analyses. The instrument has allowed our laboratory to increase productivity considerably in a way that would never have been possible before. Implementation was a very smooth process requiring minimal configuration effort and the Thermo Fisher Scientific team of experts provided valuable
support both throughout and after this process.”

For more information, visit www.thermoscientific.com/easylc





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