Fast purification of his-tagged proteins

16th June 2015

Polyhistidine tags are often used for affinity purification of his-tagged recombinant proteins expressed in E. coli, Bacillus megaterium, and other protein expression systems. 

In contrast to traditional IDA matrices, MoBiTec Ni-IDA is an optimised matrix with low density of IDA ligands. This non-saturating surface concentration of IDA eliminates almost all non-specific interactions of contaminating host proteins with the adsorbent. As a result, MoBiTec Ni-IDA provides higher target protein purity.

Features include:

• Excellent tool for routine purification of his-tagged proteins
• Purification under native and denaturing conditions
• Starting from diverse expression systems, e.g., E. coli, yeast, insect, and mammalian cells
• Maximal binding capacity: 90 mg protein per column
• Protein recovery rate >80%
• Improved target specificity by optimised silica-based Ni2+-IDA matrix
• Imidazol-free loading and washing buffer
• Columns are long-term storable when kept dry





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