Troubleshooting and optimising mutagenesis

Integrated DNA Technologies (IDT), a leader in oligonucleotide synthesis, provides in-depth protocols and troubleshooting suggestions for mutagenesis applications.

As part of the new, free-to-download IDT Mutagenesis Applications Guide, an extensive troubleshooting section makes it possible to optimise your mutagenesis experiments for the best possible resulting data. Everything from primer design, reaction setup, ligation and transformation efficiency, to specific reaction components and PCR parameters, are covered. The guide provides a useful reference tool for performing site-directed or random mutagenesis.

The Mutagenesis Applications Guide also describes IDT’s range of Ultramer Oligonucleotides, which can simplify mutagenesis experiments. These high fidelity oligonucleotides provide greater experimental flexibility than standard oligonucleotides due to their length¾they are available up to 200 bases.

To find out how to maximize the effectiveness of your gene mutagenesis, download the application guide as a PDF, or in e-pub format, which is compatible with most portable e-reader devices.

For more information, visit www.idtdna.com

Recent Issues