New dyes to enhance flow cytometry

Bio-Rad Laboratories has announced the launch of both the StarBright Blue-series and StarBright Yellow-series dyes to provide greater flexibility in conventional and full spectrum flow cytometry applications. Offering exceptional brightness with narrow excitation and emission profiles for precise resolution, the dyes expand research capabilities across the blue (488nm) and yellow (561nm) lasers.

Each dye in the Bio-Rad range of highly stable StarBright dyes is conjugated to flow validated antibodies, supporting flow cytometry panel design in immunology research. StarBright Blue dyes 580, 615, 675, 700, 765, and 810 are excitable by the 488nm laser, and StarBright Yellow dyes 575, 605, 665, 720, and 800 by the 561nm laser, with low background. Compared to PE and PE tandems, StarBright Blue dyes are true 488nm excitable dyes and StarBright Yellow dyes offer reduced excitation by the 488nm laser. They can be used in combination with other StarBright dyes and traditional fluorophores.

StarBright dyes are compatible with most experimental protocols and flow cytometers, including the Bio-Rad ZE5 Cell Analyser, without the need for special buffers. Minimal lot-to-lot variation helps to ensure reproducible and consistent staining, and the dyes are resistant to photobleaching with no loss of signal in fixation.

“The continued development of the Bio-Rad StarBright dye portfolio, now totalling 28 dyes and spanning multiple lasers, gives researchers greater choice and flexibility across multiplex flow cytometry applications, offering superior alternatives to existing dyes,” said Mike Blundell, PhD, Product Manager, Flow Cytometry, Life Science Group, Bio-Rad. 

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