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Hybridisation-based targeted sequencing

7th June 2017

Posted By Paul Boughton


Oxford Gene Technology (OGT) has made several advances in hybridisation-based target enrichment protocols that now enable researchers to prepare samples for sequencing in just one day.

Researchers can now access the high-quality results of hybridisation-based targeted sequencing with a speed comparable to that of amplicon-based approaches.

OGT’s hybridisation-based targeted sequencing delivers unparalleled coverage uniformity enabling researchers to detect low frequency variants consistently down to 1% VAF (variant allele frequency) at a read depth of >1000x.

The company’s latest protocol developments enable even more researchers to quickly and precisely focus in on regions relevant to their research - increasing throughput and confidence in results while saving on time and reagents.

OGT’s hybridisation assay enhancements improve speed and also enable lower input levels of DNA to be used.

The company has optimised its enzymes and buffers to allow pooling of library preparation steps enabling users to complete the whole enrichment protocol in less time.

This includes a short enzymatic fragmentation step, combined end-repair and adaptor ligation steps, and an optimised hybridisation of just 30 minutes for good quality DNA samples. In addition, OGT’s NGS panel optimisation yields good data with as little as 10ng of starting material*.





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