Beckman Coulter Life Sciences, laboratory automation and innovation specialists, has launched its new IR820 and IR870 infrared dye antibody conjugates, created to provide research flow cytometry labs with a low noise solution, including single laser excitation, no dye interferences, with negligible overlap with other fluorochrome emissions, and ultralow autofluorescence background.
IR820 and IR870 reagents have been designed for lineage marker staining, offering brightness to identify respective gating antigens, while leaving detection of non-infrared dyes uncompromised due to near-complete absence of spectral overlap. Lineage-gating markers can be added, or if already existing in the antibody panel, moved away from commonly used spectral ranges.
Pietro Lopriore, vice president and general manager of the Flow Cytometry Business Unit, said, “As high-parameter flow cytometry becomes more mainstream, antibody panel design gets even more crucial due to tightening complex spectral interference patterns that can reduce data quality. Our IR820 and IR870 infrared dye conjugates, with their low noise design, remove complexities and make the infrared spectral range a premium place for lineage markers, allowing for a more flexible antibody panel design. By enabling greater clarity, we’re able to uniquely empower researchers in an exciting new frontier of human phenotyping experiments.”
Current flow cytometry fluorochrome conjugates do not cover the infrared range. Lineage markers used to identify major subpopulations usually reside in the same spectral ranges as probes for weak co-expressed antigens, enabling characterisation of subpopulations and their functional status. By this spectral colocalisation, typically bright lineage markers could compromise the resolution and detection of their dimmer co-expressed companions.
Dr. Theo Gerdener, head of immune phenotyping at ABJ Oncology Pretoria, South Africa, said, “We recently tested the CD3 IR820 infrared flow cytometry antibody, and we are thoroughly impressed with the results. By using markers in these channels, we can reserve the other channels for other additional markers that are less common, which is crucial for complex multi-parameter assays. Given the clear separation and minimal interference with other channels, we highly recommend this infrared antibody for anyone looking to expand their flow cytometry panels.”
This new addition to the Beckman Coulter Life Sciences reagent portfolio is designed for users of IR-equipped CytoFLEX LX and S instruments, including those using the spectral mosaic detection module, facilitating human phenotyping within an accessible spectral range. The non-tandem dyes, IR820 and IR870, are excited at 808 nm and emit at 835 nm and 896 nm, suitable for 840/20 nm and 885/40 nm filters. These Research Use Only reagents include CD3, CD4, CD8, CD14, CD20, and CD45 and are manufactured in accordance with current good manufacturing practices.
