Key to the high throughput of this method is the use of the MicroluteTM 96-well SPE system, which allows a batch of samples to be prepared for injection in less than half the time that is actually required to analyse them. The method meets all regulatory requirements for clinical studies, and is suitable for the analysis of large numbers of samples. A copy of Application Note D may be downloaded from www.porvair-sciences.com/downloads.php.
The developed method using the MicroluteTM was validated over a concentration range of 100 to 10,000 pg /ml. Recovery using the SPE method was approximately 60%, and consistent across the calibration range. The authors describe how the MicroluteTM SPE method, unlike protein precipitation sample preparation, is free from LC/MS matrix effects that may influence the ionisation of the compound(s) of interest.
Developed in conjunction with a leading pharmaceutical company the MicroluteTM (http://www.porvair-sciences.com/acatalog/home.html) has become the benchmark for high throughput sample preparation across a range of applications. The innovative design of the MicroluteTM from Porvair Sciences Ltd offers all the advantages of automated and high throughput SPE sample preparation in a convenient microplate format capable of rapidly processing 96 samples in one go repeatedly and precisely.
Established in 1992, Porvair Sciences Ltd. expertise in microplate technology and manufacturing covers scientific fields including Life Sciences, Drug Discovery, Combinatorial Chemistry, Solid Phase Extraction, Protein Purification, High Throughput Screening, Proteomics and Genomics. Porvair Sciences Ltd. is a wholly owned subsidiary of Porvair plc.
For further information please contact Porvair Sciences Ltd. on telephone +44-1372-824290 or email int.sales@porvair-sciences.com.
