Amsbio has launched StemFit Basic05, a next-generation, animal origin-free medium designed for scalable human pluripotent stem cell (hPSC) manufacturing.
This cell culture medium is compatible with 2D adherent and 3D suspension culture, and enables a streamlined workflow from cell expansion to process scale-up using a single medium. Using a single medium across culture modes minimises the process re-development and optimisation traditionally associated with media changes.
Pluripotent stem cells cultured in StemFit Basic05 can retain their differentiation capability and have been confirmed to differentiate directly from 3D aggregates.
For labs seeking to implement stable, scalable suspension culture and processes, high cell viability and uniform spheroid formation are essential prerequisites. StemFit Basic 05 has been shown to reliably deliver high cell viability and uniform spheroid formation across multiple human iPSC lines, while maintaining a normal karyotype. With robust, highly reproducible uniform spheroid formation, StemFit Basic05 is a suitable process development medium that also reduces process complexity and development burden.
StemFit Basic05 supports flexible culture scheduling, with comparable cell expansion even when weekend feeding was omitted.
