Tag: BMG LABTECH

Box 1: Optimizing cell-based assays on a microplate reader

Lab Consumables

Utility and scale: microplate readers in action

Fig. 2. (a) Cellular generation of ROS and impact of ROS-inducing Menandione and the antioxidant N-Acetylcysteine. (b) Luminescent signal obtained from HeLa cells treated with Menandione and increasing concentrations of N-Acetyl-L-cysteine using the ROS-Glo assay

Lab Consumables

Easy assay analysis

Fig. 2. Linear relationship of percentage of GFP+/mcherry+-HeLas (=transfection efficiency) and obtained GFP signal with matrix scan. Error bars refer to 8 replicates. Matrix scan example shown for one well each (round area below the bars)

Lab Consumables

Efficiency Drive

Enzyme Kinetics Considerations

1

Deviation from the actual DNA concentration due to potential contaminations, calculated based on A260

Lab Consumables

Why DNA purity ratios matter

Fig. 2 (A) Anti-viral screening (B) Comparison of images and well-scans showing viral replication in infected cells either non-treated or treated with different drugs. Images were taken with an imaging device, well-scans were performed on a microplate reader

Lab Consumables

Virus Assays on Microplate Readers

FIG. 2. (a) Principle of BRET-based live cell assay to monitor PROTAC activity. (b) BRET assay employed to monitor the interaction of BRD4 and VHL induced by the PROTAC ARV 771

Lab Consumables

Monitoring PROTAC activity with luminescence assay

A new generation of microplate readers