Research tools for PD-1 checkpoint inhibitors

AMSBIO has launched new cell lines and controls for Programmed Cell Death Protein 1 (PD-1) research, and to test inhibitors of the PD-1/PD-L1 pathway.

The binding of PD-1, a receptor expressed on activated T-cells, to its ligands (PD-L1 and PD-L2) negatively regulates immune responses. PD-1 ligands are highly expressed in many cancers, and their interaction inhibits T-cell activity allowing cancer cells to escape immune surveillance. The PD-1: PD-Ligands pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

A PD- /NFAT Reporter - Jurkat Cell Line is now available for applications including screening for activators or inhibitors of PD-1 signalling in a cellular context and also for characterising the biological activity of PD-1 and its interactions with ligands.

AMSBIO has also introduced the accompanying TCR activator/PD-L1 - CHO Recombinant Cell line that is purpose designed for screening for activators or inhibitors of PD-1 signalling as well as screening PD-L1 antibodies for their binding affinity.

The functionality of the cell lines was validated using a PD-1:PD-L1 cell-based assay and expression of human PD-1 or PD-L1 in the cell lines was confirmed by Western blotting.

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