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Real-time analysis of complex samples

4th September 2019


Microsaic Systems, the developer of point of need mass spectrometry (MS) instruments, has developed proof of concept workflows to analyse in real-time complex samples from bioreactors, which could enable customers to achieve significant costs and time savings.

An independent laboratory was engaged to compare the use of Microsaic's MiD ProteinID technology as an in-situ technique for measuring small and large molecules during the manufacture of biologics compared with the use of traditionally accepted bioanalysers. The results were obtained by quantifying small-molecule cell media as well as measuring intact proteins. Microsaic's MS technology detected metabolites not usually seen by traditional methods during the bio-manufacturing process.

From the information obtained, Microsaic estimates that there are potentially substantial savings available for its customers by switching from traditional analysis methods to the MiD ProteinID technology.

In March the company published early results with the Massachusetts Institute of Technology (MIT) which demonstrated the real-time analysis of complex protein feedstocks: the results highlighted the importance of storage conditions between fresh fed batch and frozen perfusion samples to beneficial biologic manufacture outcomes.

Glenn Tracey, CEO of Microsaic, commented: "These latest results are extremely exciting and mark yet another important milestone in the strategy set out when I took on the role of CEO. What we have demonstrated is the potential for our technology to replace very expensive analysers, which have been the mainstay of bio-analysis over time. As our technology can measure small and large molecules simultaneously, we can potentially replace workflows which use several analysis techniques costing hundreds of thousands of pounds each in capital expenditure. Additionally, because our technology is 'label-free' (scientists do not need to tag their molecules of interest with dyes such that the current technology can identify them), Microsaic can potentially save hours of workflow time and provide a significant saving in reagent costs."

He added: "New demands on bioprocessing mean that there is a requirement for real-time information at the point of need. Our deployable MS technology samples the media from a bioreactor in real-time, providing critical information regarding process monitoring and quality attributes of the end-product. This information can then be used to maximise yields of biologics by optimising the cell media, cell feeding and harvesting strategies of the target biologic product. As a result of our technology being able to measure small and large molecules, we can further analyse those process changes for cell media, feedstocks and end-product biologics.

In addition, MS point of need analysis would provide timely safety assurance, as harmful post-translation modifications of the product, and other dangerous host cell proteins, can potentially be controlled and minimised upstream."





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