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Imaging specimens in aqueous solution

7th July 2014


Studying Vascular Development using Zebrafish (somites). Left: without correction. Right: optics adapted to the refractive index of the water column by using the correction ring of the Leica Planapo 2.0x CORR objective. Image courtesy of Mailin J. Hamm, Angiogenesis Laboratory, University of Muenster, Germany
Studying Vascular Development using Zebrafish (eye). Left: without correction. Right: optics adapted to the refractive index of the water column by using the correction ring of the Leica Planapo 2.0x CORR objective. Image courtesy of Mailin J. Hamm, Angiogenesis Laboratory, University of Muenster, Germany

Leica Microsystems’ Leica Planapo 2.0x CORR objective for the Leica M series stereo microscope is specifically for the use with specimens immersed in aqueous solution. 

With this objective, users can obtain pin sharp visualisation of specimens with up to a 5mm water column between the specimen and the objective. 

The objective solves a problem many stereo microscope users have: observing specimens in aqueous solution deteriorates image quality due to the refractive index mismatch between water and the air surrounding the microscope objective. 

With the Leica Planapo 2.0x CORR objective, interesting structures cannot be misread

As the refractive index can be compensated with the Leica Planapo 2.0x CORR objective, interesting structures cannot be misread due to aberrations any more. Image quality is further enhanced by the objective?s high numerical aperture of up to 0.35.

Many stereo microscope applications like zebra fish research, in vitro fertilisation or transgenics require aqueous solution to optimise the preparation process or to keep the specimen alive. Especially at high magnifications this poses an aberration problem, which results in blurred images with lower information content. 

The Leica Planapo 2.0x CORR objective overcomes this problem with the help of an adjustable correction ring. Turning the ring to the specified position enables users adapt the optic to the correct refractive index according to the water column above the specimen. If users wish to observe specimens in an airy surrounding, they simply return the correction ring to the home position.





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