High throughput 3D cell screening workflows

Up to thousands of spheroids can be generated and cultured per plate

Researchers are turning to INTEGRA Biosciences VIAFLO 96/384 handheld electronic pipette for rapid and precise filling of specialized 3D cell culture plates, to increase throughput and meet the demands of high volume drug discovery programs.
Specialized 3D cell culture plates, such as Corning Elplasia microplates, enable highly reproducible growth of uniform 3D cultures for downstream applications. The VIAFLO 96/384 handheld electronic pipette is the perfect liquid handling solution for this and all other 3D cell screening applications. This affordable, versatile and easy to use solution has a small footprint, making it ideal for busy or crowded laboratories, and for use in a laminar flow cabinet. Interchangeable pipetting heads provide the flexibility to accommodate assays in 24 to 1536 well formats, in volume ranges from 0.5 to 1250 µl, and protocols can be preprogrammed to reduce operator error and user-to-user variability.

Corning has developed and validated protocols for its Elplasia plates, performing cell seeding, media exchange and wash steps with the VIAFLO 96/384. Crucially, the VIAFLO 96/384 is simple to program, enabling strict control of the exact dispense height, and speed of aspiration and dispensing to avoid damaging the delicate cell spheroids, ensuring reproducible liquid transfer into each individual well of the plate, for fast, accurate and precise multichannel pipetting. Up to thousands of spheroids can be generated and cultured per plate under uniform conditions, increasing throughput at a fraction of the cost of a fully automated liquid handling robot.

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