Ceramide glycanase can be used to deglycosylate a variety of glycosphingolipids (GSLs) by cleaving the β-glycosyl linkage.
GSLs are the most abundant glycans of the vertebrate brain.
The Ludger Ceramide Glycanase Kit is particularly useful in the study of GSL storage disease, such as Gaucher’s disease and neurological diseases, eg Tay-Sachs disease.
Other conditions associated with glycolipid degradation and would require GSL analysis include GM1 gangliosidosis, Krabbe’s disease, Sandhoff, Metachromatic leukodystrophy, Saposin deficiency and Farber’s disease.
Ludger’s ceramide glycanase kit (LZ-CER-HM-KIT) contains enzyme and buffer sufficient to deglycosylate 25 samples.
The kit also contains a Monosialoganglioside GM1 substrate to be used as a positive control. Free GSL glycans can then be and analysed using the Ludger Tag labelling technology for efficient identification of their glycosylation patterns.
Ludger can also analyse GSLs in house as part of its glycoprofiling services.
