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Fast and gentle confocal microscopy

29th April 2019


The new Multiplex mode for Zeiss Airyscan 2 delivers more information in less time. Smart illumination and detection schemes allow parallel pixel acquisition for fast and gentle confocal microscopy. Researchers can now image their most challenging three-dimensional samples with high framerates in superresolution. The speed and sensitivity gain can be used to gently capture either fixed samples in larger fields of view with higher throughput and without bleaching, or dynamic processes in living specimens with minimal disturbance.

The Multiplex mode is available for the whole LSM 9 family: LSM 980 is the flexible research platform with complementary multiphoton and superresolution capabilities. LSM 900 is a very compact system that delivers image quality without complexity.
 
The new LSM 980 with Airyscan 2 is the ideal research platform for confocal 4D imaging. The entire beam path is optimized for simultaneous spectral detection of multiple weak labels with the highest light efficiency. Researchers benefit from the full flexibility of a point scanning confocal and the speed and gentleness of the sensitive Airyscan 2 detector. The new Multiplex mode combines an elongated excitation laser spot and parallel pixel readout of this area detector. This allows acquiring up to eight image lines in a single sweep. Users can gently image larger fields of view with superresolution in shorter acquisition times than ever before.

LSM 900 with Airyscan 2 is a very compact confocal microscope for high-end imaging. This system has a small footprint, concentrating on the essence of a confocal and leaving out needless complexity. It fits easily into labs or imaging facilities and is optimised for ease of use. It can be combined with Celldiscoverer 7 for automated confocal imaging with high efficiency.
 
The new Multiplex mode provides more options to fit imaging speeds and resolution to experimental needs. It uses knowledge about the shape of the excitation laser spot and the location of single area detector elements within the Airyscan 2 detector to extract more spatial information ­ even during parallel pixel readout. This allows bigger steps to be taken when sweeping the excitation laser over the field of view, improving achievable acquisition speeds. In fact, the large amount of spatial information captured in the pinhole plane allows the reconstruction of a final image with better resolution than the acquisition sampling.





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