Enhanced ultrafast laser for multiphoton imaging

Spectra-Physics has introduced InSight DS+, a widely-tuneable ultrafast laser system with enhanced power levels over its predecessor, the InSight DeepSee. This new laser system features ultrashort pulses over a very broad tuning range of 680 to 1300nm for deepest imaging, delivering continuous, fully automated tuning from a single beam. A second beam provides simultaneous dual-wavelength output at 1,041nm for multimodal imaging. The new 

InSight DS+ is ideal for in vivo deep tissue imaging for neuroscience, cell biology and other life science research.

“The highly successful InSight DeepSee improved multiphoton imaging by enabling users to seamlessly image at far greater depths, and the new InSight DS+ takes that performance to the next level,” says Julien Klein from Spectra-Physics.

The new InSight DS+ offers enhanced power levels of >1.2 W at 900nm, >1.0 W at 1,000nm and >0.7 W at 1,200nm and continuous, 680-1,300nm gap-free tuning. The short <120fs pulse widths, highest peak power levels at infrared wavelengths and integrated dispersion pre-compensation result in imaging penetration depths beyond 1mm, as successfully demonstrated in live tissue. A second synchronised beam at 1,041nm is available for advanced imaging techniques, including uncaging, dual-wavelength simultaneous imaging, second-harmonic generation (SHG), third-harmonic generation (THG), coherent anti-Stokes Raman scattering (CARS), and stimulated Raman scattering (SRS) imaging.   

This fully-automated ultrafast laser can be seamlessly tuned by software to any wavelength within seconds. The laser system also has ideal beam characteristics optimised for multiphoton imaging.

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