Deep imaging of biological samples

11th September 2014

Dedicated to multiphoton excitation microscopy, Olympus has introduced its XLPLN10XSVMP and XLSLPLN25XGMP objective lenses with an 8mm working distance and support for a large range of refractive indices (RI). The objectives enable super-deep imaging of tissues treated with the latest clearing agents as well as live-cell imaging and light sheet microscopy techniques. 

Compatible with the FVMPE-RS and FV1200MPE multiphoton microscopy systems and easily adaptable to home-build light sheet microscopes, the new objectives boast a range of features providing important benefits to researchers. Both objectives offer super-long working distances of 8mm, providing the capability and space for structural and live-cell imaging deep within large intact samples (such as whole mouse brain), especially useful in neuroscience applications. A high transmittance range of 400-1600nm extending into the infrared (IR) spectrum allows deeper imaging with minimal damage to the tissue, and is well-suited to combine conventional multicolour MPE imaging with label-free methods like third- and second-harmonic generation. A field number of 27.5 provides excellent scattered light collecting abilities and a correction collar allows compensation of refractive index mismatches. Furthermore, spherical aberrations are corrected across the visible to IR range, with additional chromatic aberration correction in the IR range.

The XPLN10XSVMP is also extremely versatile with multi-immersion capability enabling its use with water, silicone and oil as well as clearing agents such as Scaleview, Clarity and SeeDB (RI 1.33-1.52). Deep macro observation is achieved with high resolution at a large field of view (FOV – 1.8mm at 10X magnification). The XLSLPLN25XGMP also supports the latest in clearing agents such as Clarity and SeeDB for cutting-edge research techniques, aided by a high RI immersion capability of 1.41-1.52 (SeeDB requires an RI of 1.49).

These additions to Olympus’ XLPLN series of objectives extend the company’s lead in the field of multiphoton excitation microscopy. The objectives enable the interrogation of large intact samples (such as whole-brain high-resolution imaging) and structural and molecular analysis of large assembled biological systems. The large FOV provided by the objectives enhances data collection from samples treated with the latest clearing agents by several orders of magnitude and this combined with unsurpassed flexibility and correctional capabilities makes them the clear choice for researchers using MPE or light sheet microscopy techniques.





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