Glycosphingolipids (GSLs) are implicated in the pathogenesis of various diseases, including Fabry disease, Gaucher disease, Tay-Sachs disease and Sandhoff disease.
The enzyme ceramide glycanase can be used for the characterisation of GSLs as it deglycosylates a variety of GSLs by cleaving the β-glycosyl linkage.
Once free, the GSL glycans can be fluorescently labelled using LudgerTag labelling technology and then analysed to identify their glycosylation patterns.
Ludger’s ceramide glycanase kit (LZ-CER-HM-KIT) contains purified ceramide glycanase enzyme, buffer and GM1 glycolipid.
The kit is sufficient to deglycosylate 25 samples. Ceramide glycanase can be used to release glycans from GSLs to enable their characterisation.
