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Accelerating ADC development

15th December 2014


Fig. 1. The Waters Biopharmaceutical Platform Solution with Unifi.
Fig. 2. This representative report from Unifi illustrates the software’s ability to provide automated calculation of drug-to-antibody ratio (DAR), which includes total DAR and distribution per sample, as well as a summary table of average total DAR, DAR distribution (%), and mean and %RSD.

Sharon Goodkowsky presents a case study on how a biotech firm fast-forwarded work on its antibody drug conjugates (ADCs).

Igenica Biotherapeutics, a US biotechnology company with a promising anti-cancer antibody in a Phase 1 clinical trial, has innovative technology platforms to discover and develop antibody drug conjugates (ADCs) for cancer treatment. To fulfil the company’s goal of quickly moving ADC-based therapeutics out of preclinical development and into the clinic, Igenica wanted new, dedicated instrumentation to fully analyse the complexity and variation within an ADC’s three parts: the antibody; the toxic drug; and the linker that binds them together. 

As a result, Igenica deployed the Waters Biopharmaceutical Platform Solution with Unifi, which includes the Acquity UPLC H-Class system, Xevo G2-S QTof mass spectrometer, and Unifi scientific information system.

“The platform has provided a superior window of insight into the molecules we are making,” said Mark R. Flory, Ph.D., a principal scientist at Igenica. “It will help in our efforts to accelerate clinical trials with ADCs.”

Before ADCs
Igenica had existing capillary HPLC/MS technology in place to drive its targeted discovery efforts. The company had anticipated using the same for ADC analysis, but wanted to improve proteomics workflows for ADC characterisation. It sought an instrument to handle different chemistries for conjugations and linkers, and to improve its characterisation of the population of antibody conjugates once the toxic payload was added by its proprietary SNAP chemistries. Its existing ion trap and orbitrap LC/MS systems worked for small molecule and peptide qualitative analyses, but the company wanted to increase the range for analysis of intact monoclonal antibody (mAb) conjugates, and increase the capability to readily determine drug loading on ADCs. 

Ingenica wanted a quadrupole time-of-flight (QTof) MS dedicated to complex, large-molecule mixtures, including ADCs. It also wanted the ability to look at intact mass profiles of ADC species in both native and denaturing electrospray ionisation modes. This became the driving force to find a new instrument that would expand its tools for analysis and expand current and future capabilities of the organisation.

The company initially evaluated single-vendor solutions from three companies and quickly narrowed down the evaluation to only Waters and one other vendor. To make a data-driven decision, Ingneica’s chemists ran a well-characterised ADC mixture through both companies’ LC/MS systems and compared analytical results, looking at drug-to-antibody ratio (DAR) results from hydrophobic interaction chromatography (HIC) LC-UV data. The team then considered other variables, including informatics quality, speed of installation and service, and ultimately selected the Waters platform. 

Waters was selected for numerous reasons, according to Flory. The Biopharmaceutical Platform Solution with Unifi delivered results comparable to HIC and other reference data for the demo sample. 

Traditional ADC strategies yield DARs from zero to eight drugs per antibody, and it has been shown that high drug loads are lost to solubility and PK issues, while low loads can lack efficacy. Igenica needed a dedicated ADC platform to quickly determine the ratio and provide feedback to the scientists in development and the Unifi scientific information system can automate DAR calculations. The software’s flexibility enables chemists, who currently tend to rely on manual calculations, to generate those values as part of data processing and reporting during routine analysis.

Igenica’s chemists like to run sets of denatured samples (reversed-phase LC/MS) overnight and corresponding native samples (non-denaturing SEC-MS) by day. The integrated Waters solution, with its facile column switching, streamlines setup and method execution to run samples quickly. 

The new solution has provided Igenica chemists with analytical capabilities that allow them to move forward – or quickly abandon – ADC candidates as cancer therapies, which can greatly accelerate their research. 

The platform is also being applied to intact mass ADC profiling and Igenica is exploring the system’s flexibility to perform peptide mapping and glycan analysis on their biotherapeutics.

For more information visit www.scientistlive.com/eurolab

Sharon Goodkowsky is with Waters in the USA. www.waters.com/adc





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