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High productivity protocol for coating of tissue culture plates & flasks

1st April 2013


INTEGRA has released an application note that describes a new protocol using its DOSE IT peristaltic pump that significantly improves the efficiency of collagen coating of plates and flasks.

Coating of dishes or tissue culture flasks with extra cellular matrix proteins like Collagen or Fibronectin is a common procedure in laboratories involved in cell culture. However, coating large numbers of disposables is a tedious and time-consuming task. This application note describes how using a DOSE-IT peristaltic pump results in 40% time-savings compared to a regular pipette controller and at the same time helps to reduce excessive strain caused by this repetitive work.

An online video demonstrating the DOSE IT peristaltic pump protocol may be viewed at www.integra-biosciences.com/sites/pdf/Improving_the_efficiency_of_collagen_coating_V01.pdf. For a copy of this application note contact INTEGRA on +41-81-286-9530 / info@integra-biosciences.com (Europe / Asia Pacific) or +1-603-578-5800 / contact@viaflo.com (North / South America).

DOSE IT is an intuitive programmable peristaltic pump that simplifies the dispensing of cell culture media, buffers and other solutions for volumes ranging from 0.1mL to 2500mL. DOSE IT features a very small footprint and weighs only 3.5Kg making it a perfect device for bench-top or in-hood dispensing applications. Programming the DOSE IT is fast and easy through a multilingual user interface (English, Spanish, German, French and Italian). The instrument's pump head accommodates the use of different tubing diameters (1-8mm) allowing a wide range of volumes to be dispensed with optimal speed and precision. A built-in print capability supports the capture of program details for record keeping and quality control. Many accessories are available to support a variety of dispensing applications.

INTEGRA is a leading provider of high-quality laboratory tools for liquid handling, media preparation, sterilization and cell cultivation. The company committed to creating innovative solutions which fulfill the needs of our customers in research, diagnostics and quality control within the life science markets and medical industry. The INTEGRA group, comprising INTEGRA Biosciences AG, Zizers, Switzerland and the INTEGRA Biosciences US, Hudson, NH, maintains a worldwide network of over 90 independent highly trained distribution partners.




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