The Company has also launched a new range of transfer plasmids with unique signal sequences and promoters, including pOET-1 and pOET-1N that increase cloning efficiency and expression of foreign genes. OET's latest transfer plasmids and existing expression suite of flashBAC products (including flashBACGOLD, flashBAC10 and flashBACULTRA) will be available both directly from the Company and the newly appointed distributors.
pOET transfer plasmids have been designed to be smaller for improved cloning efficiency and ease of use. Both pOET-1 plasmids contain a large multiple cloning site (MCS) allowing foreign genes to be inserted in the correct orientation. AcMNPV sequences flanking the gene in the transfer plasmid's MCS allow recombination with the flashBACTM DNA to insert the expression cassette into the polyhedron (polh) locus under the control of the powerful polh promoter. Additionally, pOET-1N has been designed for high level expression of foreign genes fused to an N-terminal 6×His tag, greatly easing the purification of the recombinant protein using Ni-NTA Agarose.
"pOET-1 is compatible with any baculovirus system that utilises homologous recombination in insect cells, and will enable researchers and scientists to express high levels of foreign genes," commented James Bernard, CEO of OET. "Our expanded distributor network will allow OET to reach a broader customer base and provide increased support for existing clients, who will be able to purchase their products more quickly and with reduced shipping costs and transportation time," he added.
