Protocols for sample preparation and analysis

1st April 2013

To assist with proteomics research, Pall Life Sciences, which manufactures membranes and chromatography resins that exhibit high resolution and binding capacities with low non-specific binding for the purification and concentration of proteins, has developed a detailed application handbook containing a range of protocols for sample preparation and analysis.

Protocols detailed in the handbook include:

  • Abundant Protein Removal – including protocols for affinity chromatography resins as well as with complete kits for removal of multiple abundant proteins (albumin, IgG) in one step.
  • Protein Fractionation – protocol options include chromatography resin for affinity capture, ion exchange separation, and mixed-mode capture resins.
  • Detergent Removal – applications using SDR HyperD resin.
  • Protein Concentration and Buffer Exchange – using a range of ultrafiltration spin devices, UF plates and gel filtration media.
  • Protein Detection – including polyvinylidene difluoride (PVDF) and nitrocellulose membranes for western blotting, available as cut membranes or integrated into
  • multi-well filter plates.

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Pall Europe Limited is based in Portsmouth, UK.





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