The analysis of the complete proteome is a major interest of many researchers. Of particular importance are human bodily fluids such as serum or plasma due to their ease of accessibility and their potential for the identification of disease biomarkers.
However, a remaining challenge is the high dynamic range of the protein species, which can show differences of up to
12 orders of magnitude. In serum the primary components are serum albumin, representing 6072percent and IgG, representing 1020percent of the total protein content.
The Vivapure Anti-HSA/IgG Kit utilises a combination of a unique Anti-HSA antibody fragment and Protein G resin for specifically depleting albumin and IgG from human samples.
In contrast to traditional dye based ligands, specific removal of over 95percent human albumin from a human sample is achieved while no significant amounts of other serum or plasma proteins are bound.
Albumin and IgG depleted samples are recovered in a low salt buffer, facilitating downstream applications. The method is quick, suitable for parallel processing and offered in a ready-to-use kit. This procedure improves the resolution and detection of lower abundance sample proteins by electrophoresis, chromatography and mass spectrometry by removing up to 80percent of high abundant serum proteins.
The Anti-HSA ligand used also shows reactivity against rat and mouse albumin. As Protein G is also efficient at binding rat and mouse IgG, this one kit can be utilised for processing samples from the three most frequently encountered sample sources in biomarker research.
Vivascience AG is based in Hanover, Germany. www.vivascience.com
