Glycoprotein labeling compatible with downstream immunofluorescence
Leading innovator in fluorescent dyes and life science reagents, Biotium, has launched GlycoLiner Cell Surface Glycoprotein Labeling Kits for rapid, selective, and covalent labeling of cell surface glycoproteins.
Unlike conventional aminooxy reagents that are slow to react and require harsh buffers, additional catalysts, or high label concentrations, GlycoLiner Kits utilise a simpler and more gentle self-catalysing aminooxy chemistry for efficient labeling at neutral pH.
Surface labeling can be completed in ~20 minutes at room temperature or 4°C, supporting workflows that require low-temperature conditions to prevent protein internalisation. Labels include biotin or a selection of five bright fluorescent dyes.
GlycoLiner labeling is covalent and compatible with common fixation and permeabilisation protocols or protein extraction for capture of biotinylated glycoproteins, enabling downstream immunofluorescence staining, microscopy, flow cytometry, or biochemical analysis.
GlycoLiner also produces noticeably reduced intracellular staining in dead cells, enabling clearer visualisation of cell boundaries compared with other covalent cell surface stains that can strongly label dead cell cytoplasm.
“Biotium has launched an extensive selection of unique membrane-labeling probes that support different workflows. We are excited to provide yet another option that offers highly selective imaging of live cell surface glycoproteins that is also compatible with fixation.” said Lori Roberts, PhD, director of bioscience.
