Tecan has launched a fully automated affinity purification solution for the extraction of large biomolecules, such as proteins, antibodies and antibody-drug conjugates. This set-up takes advantage of the new Thermo Scientific MSIA Streptavidin EVO microcolumns, which have been specifically designed for use on Tecan’s Freedom EVO platform. In combination with the Freedom EVO workstation, this solution is suitabe for high throughput applications in biopharmaceutical development, clinical research and anti-doping laboratories.
Housed within a pipette tip, the proprietary MSIA microcolumns are densely coated with streptavidin for affinity purification using any biotinylated ligand, even at low concentrations. Compared with traditional resin- and bead-based methods, this new approach offers improved analytical sensitivity and lower background noise, while the automated workflow enables easier method standardisation and transfer between labs. This allows multi-analyte characterisation over a wide dynamic range of three orders of magnitude, while maintaining good reproducibility of <5%CV*.
* Antwi et al. A universal LB-MSIA workflow using Freedom EVO platform for the preclinical analysis of therapeutic antibodies of differing allotypes in rodent plasma. 2015, Thermo Fisher Scientific.
