Sartorius Stedim Biotech has announced the ambr 15 automated micro bioreactor system combined with a Nova Biomedical (Nova) BioProfile FLEX2 automated cell culture analyser.
This newly integrated system, which is a first from SSB and Nova’s successful collaboration, enables rapid at-line sample collection and analysis of massive quantities of key cell culture data.
SSB and Nova have collaborated to seamlessly integrate the software interface that controls the ambr 15 workstation for automated parallel processing of 24 or 48 single-use bioreactors (10–15mL working volume) with the cell culture analysis capabilities of the BioProfile FLEX2.
The combined system provides a unique tool to simultaneously run, sample and analyse a massive number of cell culture conditions during cell line and media development by Design of Experiments (DoE).
Combining the ambr 15 and the BioProfile® FLEX2 enables fully integrated automatic sample transfer, analysis and automated feedback control in each single-use ambr 15 bioreactor.
This allows collection of enormous quantities of cell culture data, including total and viable cell density, viability, cell diameter, pH, PCO2, PO2, glucose, lactate, glutamine, glutamate, ammonium, Na+, K+, Ca++ and osmolality, which can be sampled and measured with a complete cycle time of just 6–7 minutes.
The ambr 15 software simultaneously acquires the BioProfile FLEX2 data set and processes it, for example, by performing complex in-run calculations of how much feed volume to automatically add during a run.
This saves time by significantly increasing the amount of consistent clone selection, media development and culture parameter data that a scientist can generate per run.
