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Short guide to 3D cell culture

1st April 2013


A new informative booklet entitled ‘Short Guide to 3D Cell Culture’ has been produced by AMSBIO. Drawing upon a wealth of experience of working with a wide range of 3D cell culture procedures and formats the guide is aimed at assisting research scientists looking to culture cells in more physiologically relevant environments.

Traditional monolayer cell culture on plastic dishes or in flasks, rarely recreates the conditions cells experience in vivo. Over the past 20 years, many researchers have actively sought ways to culture cells in 3D. The conclusion reached by many researchers is that there is no silver bullet, each cell type needs a different environment and many biological questions may need specific methods to study them.

The AMSBIO guide provides background information on what is 3D cell culture and why researchers are increasingly switching to it.  Many types of 3D cell culture matrices for spheroid, organotypic and directional cultures are described in addition to natural hydrogels, scaffold-free cell culturing, artificial scaffolds and synthetic biomimetics. An up-to-date summary details how 3D cell culture techniques are helping to advance cellular research in applications including viability and cytotoxicity, invasion and motility, cell signaling and communication as well as tissue regeneration and stem cell growth and differentiation. An easy-to-use table is included to guide researchers towards the optimal 3D cell culture matrix for different applications.

For a copy of this new guide please visit www.amsbio.com/Guide-to-3D-Cell-Culture.aspx  or contact AMSBIO on +44-1235-828200 / +1-800-987-0985 or email 3Dcellculture@amsbio.com.

AMSBIO offers many matrices and other solutions for 3D culture. The company also offers expert consultative advice and in-house 3D culture services drawing upon AMSBIO's extensive bio-repository to find specific cells or tissues to culture.

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized today as a leading company contributing to the acceleration of discovery through the provision of cutting-edge life science technology products and services for research and development in the medical, nutrition, cosmetics and energy industries. The AMSBIO range includes specialist antibodies, peptides and recombinant proteins. In addition the company is able to draw upon in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. Widely acknowledged as experts in cell culture, AMSBIO partners with clients in tailoring cell systems to enhance screening outcomes and eventual prognosis. With a range of molecular detection reagents, and a significant Biorepository the company can also provide tissue DNA, RNA, protein and microarray products. Key research areas for these products include: Oncology, Regenerative Medicine, Environmental Analysis, Cytotoxicity Screening, Glycomics and Stem Cell Biology.






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